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Migration of HUVEC were evaluated by a Transwell assay using a 24-well, 8-μm-pore size Transwell plate (Costar, Cambridge, MA). HUVEC (1.5 × 105 cells/well) were seeded in the upper chamber. The lower side of the chamber was filled with CM from the HepG2 cell lines following DP treatments. After 48 h incubation, the migrated cells were stained by 0.1% crystal violet. Migrated cells were photographed by a microscope (Olympus BX51).
Copyright and License information: ©2020 Ren, Wu, Yang, Yang, Wang and Li
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