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Isolation and identification of RVFV and SBV-specific VHHs

PS Paul J Wichgers Schreur
SW Sandra van de Water
MH Michiel Harmsen
EB Erick Bermúdez-Méndez
DD Dubravka Drabek
FG Frank Grosveld
KW Kerstin Wernike
MB Martin Beer
AA Andrea Aebischer
OD Olalekan Daramola
SC Sara Rodriguez Conde
KB Karen Brennan
DK Dorota Kozub
MK Maiken Søndergaard Kristiansen
KM Kieran K Mistry
ZD Ziyan Deng
JH Jan Hellert
PG Pablo Guardado-Calvo
FR Félix A Rey
LK Lucien van Keulen
JK Jeroen Kortekaas
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After the second or third round of panning and transduction to E. coli TG1 cells, individual colonies were picked and the VHH expression was induced with 3 mM IPTG. Soluble VHHs, extracted from the periplasm, were tested for binding to the RVFV-Gnecto and SBV-Gchead antigens at 10-fold dilution as described below (ELISA). Individual VHHs were sequenced and aligned according to the IMGT system as described (Harmsen et al., 2000; Lefranc et al., 2003).

Copyright and License information:  ©2020, Wichgers Schreur et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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