Levels of interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) in the colon homogenates were measured using monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) (Rat IL-6 DuoSet ELISA, Rat IL-1β DuoSet ELISA, and Rat TNF-α DuoSet ELISA; R&D, Minneapolis, MN, USA) as specified by the manufacturer.
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