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Quantification of osteoblast differentiation by alkaline phosphatase staining

TZ Taifeng Zhou
BG Bo Gao
YF Yi Fan
YL Yuchen Liu
SF Shuhao Feng
QC Qian Cong
XZ Xiaolei Zhang
YZ Yaxing Zhou
PY Prem S Yadav
JL Jiachen Lin
NW Nan Wu
LZ Liang Zhao
DH Dongsheng Huang
SZ Shuanhu Zhou
PS Peiqiang Su
YY Yingzi Yang
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Osteoblast differentiation was assayed by alkaline phosphatase (ALP) staining and quantified with ImageJ software as previously described (Dupont et al., 2011). For each sample, at least five low magnification (X20) pictures were taken for each sample, and osteogenic differentiation was quantified by the ALP+ area determined with ImageJ as the number of blue pixels across the picture. This value was normalized to the number of cells (Hoechst/nuclei) for each picture (arbitrary units).

Copyright and License information:  ©2020, Zhou et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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