2.3. MTT Assay

MTT assays were carried out to determine the cell viability post treatment with the drug(s). Briefly, ~5000 cells were cultured in 96-well plates followed by synchronization in a serum free medium overnight. The viability of the cells was determined at 72 h post exposure to drug(s) with the MTT solution (5 mg/mL for 3–4 h at 37 °C). DMSO was used to solubilize the formazan crystals and the optical density (O.D.) was measured at 540 nm with µQuant spectrophotometric plate reader (Bio-Tek; Seattle, WA, USA).