Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Qualitative Analysis of Damage of the Terminal Ileum

CH Cathelijne Heymans
IL Ilse H. de Lange
MH Matthias C. Hütten
KL Kaatje Lenaerts
NR Nadine J. E. de Ruijter
LK Lilian C. G. A. Kessels
GR Glenn Rademakers
VM Veerle Melotte
WB Werend Boesmans
MS Masatoshi Saito
HU Haruo Usuda
SS Sarah J. Stock
OS Owen B. Spiller
MB Michael L. Beeton
MP Matthew S. Payne
BK Boris W. Kramer
JN John P. Newnham
AJ Alan H. Jobe
MK Matthew W. Kemp
WG Wim G. van Gemert
TW Tim G. A. M. Wolfs
request Request a Protocol
ask Ask a question
Favorite

H&E slides were analyzed by two independent investigators blinded to the experimental setup to assess damage of the terminal ileum. A scoring system from 0 to 4 was used to describe the severity of histological injury. Scoring was as follows: 0, no damage; 1, disrupted epithelial lining, but no loss of enterocytes; 2, disrupted epithelial lining, mild enterocyte loss from the villus tips; 3, disrupted epithelial lining, moderate enterocyte loss from villus tips, some debris in the lumen; and 4, abundant enterocyte loss from villus tips, abundant debris in the lumen, and severe shedding of villus tips.

Copyright and License information:  ©2020 Heymans, de Lange, Hütten, Lenaerts, de Ruijter, Kessels, Rademakers, Melotte, Boesmans, Saito, Usuda, Stock, Spiller, Beeton, Payne, Kramer, Newnham, Jobe, Kemp, van Gemert and Wolfs.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A