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Motility assays.

LH Louie D. Henderson
TM Teige R. S. Matthews-Palmer
CG Connor J. Gulbronson
DR Deborah A. Ribardo
MB Morgan Beeby
DH David R. Hendrixson
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C. jejuni strains were suspended from plates in MH broth and diluted to an OD600 of 0.8. Each bacterial strain was stabbed into semisolid MH motility medium containing 0.4% agar using an inoculation needle and then incubated for 30 h at 37°C under microaerobic conditions. When appropriate, motility agar contained chloramphenicol or kanamycin to maintain plasmids for in trans complementation of mutants. For dark-field microscopy, the cultures were further diluted 1:10 in MH broth or MH broth with 0.35% methylcellulose. Strains were immediately analyzed for motility by applying 3 μl of culture between a glass slide and glass coverslip.

Copyright and License information:  ©2020 Henderson et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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