2.10. PAK1 inhibition by small‐molecule inhibitors

The PAK1 small‐molecule inhibitor FRAX‐597 (S7271) was purchased from Selleck Chemicals (Houston, TX, USA), and G‐5555 (HY‐19635) was purchased from MedChemExpress, LLC (Monmouth Junction, NJ, USA). DMSO was used as a vehicle control. For drug efficacy evaluation, 1 µm of FRAX‐597 or G‐5555 was incubated with the cell lines for 3 h. For migration assays, 1 µm of each drug was incubated with the starved cells before the assay. Migrated cells were stained and counted after 3 h of incubation.