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Nitrogenase Activity Assay

LY Li Yang
SM Sarra El Msehli
SB Sofiane Benyamina
AL Annie Lambert
JH Julie Hopkins
JC Julie Cazareth
OP Olivier Pierre
DH Didier Hérouart
SA Samira Achi-Smiti
EB Eric Boncompagni
PF Pierre Frendo
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Nitrogen fixation activity was determined by C2H2 reduction assay (ARA), using a gas chromatograph. Nodulated roots at 10 and 20 dpi were detached from plants and put inside sealed vials. In each vial, 10% of the atmosphere was substituted with acetylene. After 1 h of incubation at 28°C, gas samples were analyzed with a gas chromatograph system (Agilent GC 6890N; Agilent Technologies, Massy, France). Twelve plants were tested per replicate and at least three different biological replicates were analyzed.

Copyright and License information:  ©2020 Yang, El Msehli, Benyamina, Lambert, Hopkins, Cazareth, Pierre, Hérouart, Achi-Smiti, Boncompagni and Frendo
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