Total antioxidant activity (FRAP assay)

Pavithra Mettupalayam Kaliyannan Sundaramoorthy; Kannan Kilavan Packiam

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Total antioxidant activity (FRAP assay)

PS Pavithra Mettupalayam Kaliyannan Sundaramoorthy

KP Kannan Kilavan Packiam

This method is extracted from research article: BMC Complement Med Ther, Apr 2020

In vitro enzyme inhibitory and cytotoxic studies with Evolvulus alsinoides (Linn.) Linn. Leaf extract: a plant from Ayurveda recognized as Dasapushpam for the management of Alzheimer’s disease and diabetes mellitus

DOI: 10.1186/s12906-020-02922-7

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The antioxidant competence of the extracts was evaluated using 2,4,6-tripyridyl-S-triazine (TPTZ) in FeCl₃·6H₂O solution. Reduction of colourless Fe³⁺ TPTZ complex to blue-coloured Fe²⁺-tripyridyltriazine at low pH by the presence of electron-donating antioxidants was determined. This reaction was measured by computing the absorbance at 593 nm. The constituents of Ferric Reducing Antioxidant Power (FRAP) reagent are 300 mM acetate buffer, 10 mL TPTZ in 40 mM HCl and 20 mM FeCl₃.6H₂O in the ratio of 10:1:1 at 37 °C. For each reaction, Diluted plant sample (5 μL) of was added to 3995 μL fresh FRAP reagent and mixed using a vortex mixer and incubated for 30 min at 37 °C. The absorbance was read at 593 nm with ascorbic acid as standard [26]. The reducing potential of the extracts was measured, and IC₅₀ value was determined for each extract.

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