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Southern Blot Hybridization

SG Sònia Garcia
JW Jonathan F. Wendel
NB Natalia Borowska-Zuchowska
MA Malika Aïnouche
AK Alena Kuderova
AK Ales Kovarik
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Total genomic DNA was extracted from fresh young leaves using a modified CTAB method following procedures described previously (Kovarik et al., 1997). Genomic DNA was digested with the BamHI restriction enzyme and hybridized on blots. The DNAs were digested with BamHI and hybridized with the radioactively labeled ([32P] dCTP, Dekaprimer labeling kit (Thermo Fischer, USA) 5S rDNA probe. The probe was a trimer of the 5S rRNA gene from Artemisia tridentata [S4 clone, GenBank # JX101915.1, (Garcia et al., 2012b)]. Hybridization signals were visualized using a PhosphorImager (Fuji, FLA 9000).

Copyright and License information:  ©2020 Garcia, Wendel, Borowska-Zuchowska, Aïnouche, Kuderova and Kovarik
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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