Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

B16F10 mouse model of melanoma

CM Carmela Martini
ET Emma J. Thompson
SH Stephanie R. Hyslop
MC Michaelia P. Cockshell
BD Brian J. Dale
LE Lisa M. Ebert
AW Anthony E. Woods
EJ Emma C. Josefsson
CB Claudine S. Bonder
request Request a Protocol
ask Ask a question
Favorite

For syngeneic studies, 7–8 week-old female or male, wildtype or Bcl-xPlt20/Plt20 mice41 on C57BL/6 background were used78. 1 × 106 B16F10 cells in 50% growth factor-reduced Matrigel were injected subcutaneously into the flank. Caliper measurements of the tumours were taken every 2–3 days and after the animals were euthanized, their primary tumour, lungs and liver were harvested for histology. Blood was collected pre- and post- experiment from the retro-orbital sinus into Microtainer tubes containing EDTA, and circulating platelet and white blood cells (WBC) counts were performed using an ADVIA 2120 haematological analyser (Seimens, Munich Germany). Mice were rested for 2 weeks following bleeding prior to tumour cell injection.

Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A