2.10. Tumor xenograft model

Female BALB/c nude mice (4–6 weeks of age) were used and assigned randomly to two groups (five mice in each group): sh‐NC and sh‐RACK1. A human OSCC tumor model was established by subcutaneously injecting 1 × 10⁶ cells (0.1 mL) into the right upper flanks of the mice. From the fresh tumor tissues, single cells were isolated using collagenase IV and then stained with CD206, CD11b and F4/80 antibodies for 30 min at 4 °C. The stained cells were washed and resuspended in PBS/0.1% bovine serum albumin plus azide. Flow cytometry was performed and the results were analyzed by flowjo software.