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Bacteria were exposed to various stresses for 3 h and collected. Cells without exposure to any stress were used as the control group. Total RNA in each group was isolated using an RNAiso plus Kit (Takara, Shiga, Japan) according to the manufacturer’s instructions, and each RNA was then treated with Takara recombinant DNase I. The RNA was finally reverse transcribed using a Takara RT master mix kit by incubation for 15 min at 37°C.
Copyright and License information: ©2020 Wang, Huang, Zeng, Peng, Xu and Zhou.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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