2.6. Splenocyte Isolation and Culture

Spleens of mice were removed aseptically. To obtain single-cell suspensions, red blood cells were removed using red blood cell lysis buffer. The isolated splenocytes were incubated in the presence or absence of anti-CD3 antibody (0.5 μg/mL) (eBioscience, San Diego, CA, USA) for 24 h. After incubation, the supernatants were collected to determine the levels of IL-4, IL-5, IL-13, and IFN-γ.