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Infarct Size Measurement

XD Xiangnan Du
JY Jian Yang
CL Cuiying Liu
SW Sainan Wang
CZ Chencheng Zhang
HZ Heng Zhao
HD Huishan Du
XG Xiaokun Geng
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2,3,5-triphenyl-2H-tetrazolium chloride (TTC) staining was used for measuring the infarct size 48 h after reperfusion when the animals were euthanized. The non-ischemic hemisphere and the non-ischemic region in the ischemic hemisphere were measured, from which the infarct size was calculated according to the following formula: [(area of the non-ischemic hemisphere—area of the non-ischemic region in the ischemic hemisphere)/area of the non-ischemic hemisphere] × 100%. The detailed protocol has been described previously (Ren et al., 2009; Yang et al., 2018).

Copyright and License information:  ©2020 Du, Yang, Liu, Wang, Zhang, Zhao, Du and Geng.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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