Phagosomal pH was quantified by measuring fluorescence intensity of pH-sensitive BioParticles ({"type":"entrez-protein","attrs":{"text":"P35361","term_id":"548429","term_text":"P35361"}}P35361, Thermo Fisher, USA), which has reported previously69. Briefly, the pH dependent fluorescence curve was acquired by immersing pH-sensitive beads in standard buffers with serial pH gradient (pH 2–7). The pH-sensitive beads from buffers of different pH were loaded into confocal dishes. The image was obtained by Leica LP8X confocal microscope (Germany) and the fluorescence intensities were calculated using the LAS X Small 3.3.0 software. The data was analyzed and visualized via Graphpad Prism 8. The final quantification was averaged from four replications, and each replication contains 30 fluorescence beads.
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