pH dependent fluorescence curve

FM Fan Mao
HM Huawei Mu
NW Nai-Kei Wong
KL Kunna Liu
JS Jingchen Song
JQ Jianwen Qiu
YL Yue Lin
XZ Xiangyu Zhang
DX Duo Xu
ZX Zhiming Xiang
JL Jun Li
YZ Yang Zhang
ZY Ziniu Yu
request Request a Protocol
ask Ask a question
Favorite

Phagosomal pH was quantified by measuring fluorescence intensity of pH-sensitive BioParticles (P35361, Thermo Fisher, USA), which has reported previously69. Briefly, the pH dependent fluorescence curve was acquired by immersing pH-sensitive beads in standard buffers with serial pH gradient (pH 2–7). The pH-sensitive beads from buffers of different pH were loaded into confocal dishes. The image was obtained by Leica LP8X confocal microscope (Germany) and the fluorescence intensities were calculated using the LAS X Small 3.3.0 software. The data was analyzed and visualized via Graphpad Prism 8. The final quantification was averaged from four replications, and each replication contains 30 fluorescence beads.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A