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Confluent BHK-21 monolayers were inoculated with serial dilutions of virus suspensions for 45 min under standard culture conditions (37°C, 5% CO2). Then, monolayers were overlaid with 1× DMEM supplemented with 2% FBS and 0.6 to 0.7% agar and incubated for 20 to 24 h. Cells were then fixed with 10% formaldehyde, the agar overlay was removed to stain cells with 2% crystal violet in 10% formaldehyde, and plaques were counted.
Copyright and License information: ©2020 Andreu-Moreno and Sanjuán.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
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