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MitoTracker Red CMXRos Staining
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Cells were seeded onto glass slides in 12-well plates and treated with different compounds for 24 h. Cells were then stained with MitoTracker Red CMXRos (200 nM). After 20 min, the cells were rinsed with PBS and observed under a laser confocal scanning microscope equipped with a 63 × objective lens and an argon laser (excitation wavelength: 555 nm). Images were captured for analyzing fluorescent intensity with ImageJ software.
Copyright and License information: ©2020 Wang, Gong, Zhan, Chen, Yin, Lu, Zhang, Wang, Ke, Du, Liu and Xiao.
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