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LysoTracker Red Staining
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Cells were seeded onto glass slides in 12-well plates and treated with different chemicals for 24 h. Cells were then loaded with LysoTracker Red (50 nM, L7528, Thermo, Waltham, MA, United States). After 20 min, the cells were rinsed with PBS and observed under a laser confocal scanning microscope (excitation wavelength: 555 nm). Images were captured for analyzing the fluorescent intensity with ImageJ software.
Copyright and License information: ©2020 Wang, Gong, Zhan, Chen, Yin, Lu, Zhang, Wang, Ke, Du, Liu and Xiao.
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