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Mushroom tyrosinase inhibitory activity was determined using the following method. A reaction mixture was prepared with 125 μL of 0.1 M sodium phosphate buffer (pH 6.5), 5 μL of plant sample extract in 80% methanol (10 mg mL− 1), 30 μL of mushroom tyrosinase (1000 unit mL− 1), and 40 μL of 1.5 mM L-tyrosine, and was added to 96-well plates. The reaction mixture was incubated at 37 °C for 20 min and absorbance was measured at 490 nm. The data were presented as the mean value of three analytical replicates.
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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