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Determination of the estrous cycle phases
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The characterization of each phase of the estrous cycle in female rats was based on the three types of cells in vaginal secretion: epithelial, corneal, and leukocytes [17]. Vaginal secretion was collected using a plastic pipette containing 10 μL of saline solution introduced superficially into the vagina of the rat. This drop of saline solution was collected and placed in a glass blade for observation in an optical microscope (magnified by × 400). Measurement was performed in the ovulatory (estrus) and non-ovulatory (diestrus) phases of the estrous cycle.
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