The live/dead staining was performed 48 hours after cell seeding as was described previously61. Hoechst 33258 (Sigma) dissolved in PBS and propidium iodide (PI) solution (Life Technologies) were added directly to cell culture medium at final concentrations of 2 μg/ml and 5 μg/ml respectively. Cells were incubated for 2 hours and then immediately examined with an inverted fluorescence microscope.
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