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We carried out the assay in male mice at 6–10 weeks of age, as described previously25. We suspended RENCA cells (2 × 106) in 150 μl of phosphate-buffered saline (PBS) and injected them into a chamber comprising a ring covered with Millipore filters (0.45 μm pore size) (P1688; Merck Millipore, Darmstadt, Germany) on each side. On day 5, we removed the chambers from the subcutaneous fascia and replaced them with black rings with the same inner diameter as the chambers. We assessed photographs of these sites by counting the number of newly formed vessels.
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