Masson’s Trichrome Staining and Histological Analysis

After extraction of all mice hearts, they were washed with PBS, fixed, embedded, and sectioned into 5 μm sections. Masson’s trichrome staining was performed according to instructions of the trichrome stain kit (Sigma-Aldrich, St. Louis, MO, USA). The infarct size, fibrosis, and scar thickness were quantified with ImageJ software (NIH, Bethesda, MD, USA).