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The Beckman Coulter method was used to assess cell viability using a ZTM Series Coulter Counter (Beckman Coulter Inc., CA). Briefly, the differentiated monolayers were exposed to GO or GNPs at 0, 5, 10, 25, 50 and 100 μg/mL for 24 h. Subsequently, monolayers were washed with PBS twice, trypsinized for 5 min at 37 °C, and diluted 1:100 in ISOTON (Beckman Coulter Inc., CA). Finally, the detached cells were counted with a Beckman Cell Counter. Two independent experiments (n = 2) were performed.
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