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Plasma prostaglandin E2 (PGE2) measurement
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An ELISA assay was performed to measure plasma PGE2 levels of the patients using DetectX Prostaglandin E2 Enzyme Immunoassay kit (Arbor Assays, MI, USA) according to the manufacturer’s directions. Kruskal-Wallis with post-hoc Dunn test adjusted by Benjamini-Hochberg FDR method was used to compare the PGE2 levels between test groups and control group. Data was represented in median. p < 0.05 was considered statistically significant.
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