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HUVECs were incubated for 30 min with 25 µmol fluorescent probe CM-H2DCFDA, then washed twice with PBS. To detect ROS inside cells, a multi-well fluorescent spectrophotometer was used at absorbance 485–530 nm. The intensity of generation of ROS in control group was manually set at 100%.
Copyright and License information: ©2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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