Cells were plated in culture medium in 96-well plates at 3 × 103 cells/well. After 24 h, cells were treated with axitinib, and assays performed at 24, 48, and 72 h. For cell viability assays, cells were stained with 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Amresco, Solon, OH, USA); after 4 h of additional incubation, the medium was discarded, 100ul of acidic isopropanol (0.1 N HCL in absolute isopropanol) was added, and the plate was shaken gently. Absorbance was measured on an enzyme linked immunosorbent assay (ELISA) reader at a wavelength of 540 nm. Experiment was conducted as our previous study23.
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