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AP site incision assay
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Oligonucleotide containing a synthetic analogue of an AP-site 3-hydroxy-2- hydroxymethyltetrahydrofuran (shown as X) was annealed to its complementary oligonucleotide. The AP-site incision reaction was carried out in 20 mM HEPES-KOH pH 7.5, 50 mM KCl, 5 mM MgCl2, 1 mM DTT, 0.36 mg/ml BSA, 0.5% glycerol, using 1 μg extract and 2 pmol DNA substrate, at 30 °C for 5 min.
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