Isolation, culture, and differentiation of urine-derived renal progenitor cells

MR Md Shaifur Rahman
WW Wasco Wruck
LS Lucas-Sebastian Spitzhorn
LN Lisa Nguyen
MB Martina Bohndorf
SM Soraia Martins
FA Fatima Asar
AN Audrey Ncube
LE Lars Erichsen
NG Nina Graffmann
JA James Adjaye
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Urine samples were collected from 10 healthy donors with diverse age, gender and ethnicity (Supplemental Table S1). Isolation and expansion of the urine-derived renal progenitors followed the previously established protocols37,41. For differentiation of the urine derived renal progenitors, 10 µM CHIR99021 was added to the cell culture medium for 2 days. Adult kidney biopsy derived primary human renal epithelial cells (hREPCs) (C-12665, Promo Cell, Germany) were used as control. To inhibit FGF signaling in urine derived renal progenitors, 15 µM SU5402 was added to the cell culture medium for 2 days.

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