Reagents and Antibodies

rhFGF21 was supported from Key Laboratory of Biopharmaceutical, School of Pharmaceuticals Sciences, Wenzhou Medical University, that is produced and purified from Escherichia according to the reference (Wang et al., 2010). FGFR1 inhibitor PD173074 was purchased from Selleckchem (Houston, TX, USA). LPS was obtained from Sigma (Sigma-Aldrich, St Louis, MO). The following primary antibodies applied were purchased from Abcam (Cambridge, MA, USA): anti-FGFR1 (No. ab824), anti-p-FGFR1 (No. ab59194), and anti-β-actin (No. ab8227); anti-NF-κB (No. 8242) and anti-p-NF-κB (No. 3033s) were purchased from Cell Signalling Technology (Danvers, MA, USA); anti-BDNF (No. BS9896M) and anti-Iba1 (No. 019-19741) were purchased from Bioworld technology (Louis Park, MN, USA) and FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), respectively. The secondary antibodies used in this study were goat anti-rabbit IgG H&L (HRP) (No. ab6721) and Alexa Fluor ^®488-conjugated donkey anti-rabbit (No. ab150073) purchased from Abcam (Cambridge, MA, USA).