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HeLa cells cultured in serum-free medium were treated with CK (5 nM) or DMSO for 1 day. Cells and supernatant were then collected and centrifuged, with the cell pellet resuspended in 195 µL binding buffer (Beyotime, C1062S). Cells were later stained with the FITC-Annexin V apoptosis detection kit (Beyotime, C1062S) according to manufacturer’s instructions, and analyzed by flow cytometry using the CytoFLEX S (BECKMAN COULTER).
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