Hoechst 33258 staining assay

YY Yuanyuan Yang
XL Xiaojian Liu
SL Shuang Li
YC Yanhao Chen
YZ Yongxu Zhao
YW Yuda Wei
YQ Yan Qiu
YL Yan Liu
ZZ Zhihua Zhou
JH Jun Han
GW Guohao Wu
QD Qiurong Ding
request Request a Protocol
ask Ask a question
Favorite

Hoechst 33258 (ThermoFisher, H3569) staining was performed to capture apoptotic induction of CK to HeLa cells. HeLa cells cultured in serum-free medium were treated with CK (5 nM) or DMSO for 1 or 2 days, before fixed with 4% paraformaldehyde for 30 min at 4 °C. Cells were then stained with Hoechst 33258 solution for 10 min at room temperature and subjected to imaging using a fluorescence microscope (Olympus BX53).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A