Hoechst 33258 (ThermoFisher, H3569) staining was performed to capture apoptotic induction of CK to HeLa cells. HeLa cells cultured in serum-free medium were treated with CK (5 nM) or DMSO for 1 or 2 days, before fixed with 4% paraformaldehyde for 30 min at 4 °C. Cells were then stained with Hoechst 33258 solution for 10 min at room temperature and subjected to imaging using a fluorescence microscope (Olympus BX53).
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