Cotinine (5S-1-methyl-5-(3-pyridyl) pyrrolidine-2-ona) was obtained from Sigma-Aldrich (Saint Louis, MO, United States). α4β2nAChR inhibitor dihydro-beta-erythroidine (DHβE) and the α7nAChR inhibitor, methyllycaconitine (MLA) were obtained from Tocris bioscience (Bristol, United Kingdom).
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