Materials

KLX with a purity of 99% was provided by department of pharmaceutical chemistry (College of Pharmacy, Harbin Medical University). Spontaneous hypertensive rats (SHR, 8 weeks old) were purchased from Beijing Vital River Laboratory Animal Technology Co. (Beijing, China) (Gu et al., 2016). Carbachol (CCH), N′-Nitro-L-arginine-methyl ester hydrochloride (L-NAME), indomethacin (Indo), glibenclamide (Gliben), LY294002, compound C were purchased from Sigma-Aldrich Co. Ltd. (St Louis, USA). Phenylephrine (PE) and tetraethylammonium chloride (TEA) was purchased from Aladdin (Shanghai, China). EGTA was purchased from Solarbio Life Science (Beijing, China). Griess assay kit, DAF-FM DA fluorescent probe, bicinchoninic acid protein assay reagent kit (BCA kit) were purchased from Beyotime Biotechnology (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) was purchased from Sciencell Research Laboratories (San Diego, CA, USA). (Beyotime, Shanghai, China). Primary antibodies for eNOS, phospho-eNOS ser1177, AMPKα1, phospho-AMPKα1, AKT, phospho-AKT antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). TBS buffer powder was purchased from Boster Biological Technology (Wuhan, China).