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GO function analysis and KEGG pathway analysis

WH Wenjing Han
FY Feng Yang
ZW Zhihong Wu
FG Fuqiang Guo
JZ Junjie Zhang
EH Erhan Hai
FS Fangzheng Shang
RS Rui Su
RW Ruijun Wang
ZW Zhiying Wang
ZL Zhihong Liu
YZ Yanhong Zhao
ZW Zhixin Wang
YZ Yanjun Zhang
JL Jinquan Li
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GO function analysis and KEGG Pathway analyses were carried out for the selected genes related to secondary follicle development. On the one hand, functional annotations were made for them; on the other hand, we hope to find out the main relevant functions of these candidate genes. GO function analysis is a standardized system of gene function annotation. First, differentially expressed genes are mapped to the related GO database (http://www.geneontology.org/) of each term, and the number of genes in each term is calculated. A GO function gene list and the number of the statistics are also determined. In total, GO has 3 ontologies, which respectively describe the molecular function, cellular component and biological process of genes. Pathway-based analysis is helpful to further understand the biological function of genes. KEGG is the main public database on Pathway4. GO and KEGG Pathway analysis were performed using the OmicShare tools, a free online platform for data analysis (www.omicshare.com/tools).

Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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