Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
B cells were isolated using the human B cell isolation kit II (Miltenyi Biotech, Bergisch Gladbach, Germany). Isolated B cells were stimulated with LPS (10 μg/ml) or CpG ODN2006 (10 μg/ml) alone or combined with human CD40L (1 μg/ml) for either 24 h (for co-culture experiments) or 72 h (for recovery of supernatants) at 37°C and 5% CO2.
Copyright and License information: ©2020 Busse, Campe, Redlich, Oettel, Hartig, Costa and Zenclussen.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
