Sample and Design

This cross‐sectional study was designed to assess associations of total sitting time and patterns of sitting time with cardiometabolic biomarkers in overweight/obese postmenopausal women. Archival data used in the current analysis were combined from 3 separate studies that used identical accelerometers, accelerometer wear protocols, and accelerometer data processing protocols. Signed informed consent was obtained from all participants enrolled in each of the 3 studies. Data from women enrolled in the following 3 clinical studies who were aged ≥55 years and had a body mass index (BMI) at least 25 kg/m² were included (n from each parent study included in our analysis is noted): Community of Mine (a cross‐sectional study of community‐living people residing in San Diego County)16 (n=128) and 2 randomized control trials, The MENU (Metabolic, Exercise and Nutrition at University of California, San Diego [UCSD]) study17, 18 (n=95) and The Reach for Health study19 (n=295). Women with diabetes were ineligible for the MENU and Reach for Health studies. Women with type 2 diabetes (but not type 1 diabetes) were eligible for the Community of Mine study, but none included in the current analysis who were insulin users (n=6) had taken insulin the morning of their fasting blood draw. Data used in the current analysis were collected at baseline timepoints for the 2 randomized control trials and, from all 3 studies, were critical for enrollment of participants, which resulted in low missingness (see footnote in Table 2). All studies have undergone review and approval through UCSD Institutional Review Board.