Immunoblotting and protein quantification

AS Anna Mary Steitz
AS Alina Steffes
FF Florian Finkernagel
AU Annika Unger
LS Leah Sommerfeld
JJ Julia M. Jansen
UW Uwe Wagner
JG Johannes Graumann
RM Rolf Müller
SR Silke Reinartz
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Immunoblots were performed according to standard western blotting protocols using the following antibodies: α-TGFBI (5601, Cell Signaling, Danvers, MA, USA), α-TNC (10000035, Merck), α-β-Actin (A5441, Sigma Aldrich), α-rabbit IgG horseradish peroxidase (HRP)-linked AB (27, Cell Signaling), and α-mouse IgG HRP-linked AB (32, Cell Signaling). Imaging and quantification was done using the ChemiDoc MP system and Image Lab software version 5 (Bio-Rad, Hercules, CA, USA).

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