2.3. Histological Analysis

Histological analysis of testicular sections was performed as previously described [21]. Briefly, mouse testes were fixed in Bouin’s solution for 8 h. After dehydration, tissue samples were embedded in paraffin (Leica, Mannheim, Germany). Paraffin-embedded tissue was then cut into 4 μm slices by a microtome (Leica RM2235, Mannheim, Germany). Sections were deparaffinized, rehydrated, and stained with hematoxylin and eosin (H&E). Images were examined using a microscope (Nikon ECLIPSE E200, Tokyo, Japan) and captured by Charge Coupled Device (CCD) (MshOt MS60, Guangzhou, China).