Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Histone Deacetylase Inhibitors With THP-1 and RAW264.7 Cells
Request a Protocol
Ask a
question
Favorite
To determine CP-mediated downregulation of H3K27Ac expression and induce the decrease of IL-9, cells were treated with CP or CP plus specific inhibitor (0.1 μmol/L of TSA or 1 mmol/L of VPA) for 24 h. Cells were harvested for protein or RNA isolation. Cells and supernatants were harvested and subjected to cytokine analysis.
Copyright and License information: ©2020 Jiang, Yuan, Zhu, He, Ge, Tang, Xu, Hu, Huang and Ma.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
0/150
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question0 Q&A
