Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Cytokine and chemokine levels were measured using a mouse cytokine antibody array (Mouse Cytokine Array C1, RayBiotech Inc.™) as described previously (Vikhe et al., 2019). Changes in IL-7 and IL-15 were analyzed by Mouse IL-7 and IL-15 DuoSet ELISA (R&D systems™) according to the manufacturer's instructions. The color change was measured at 450 nm on an Epoch BioTek™ plate reader and the IL-7 and IL-15 concentrations were calculated by using the standard curve obtained.
Copyright and License information: ©2020 Vikhe, Tateossian, Bharj, Brown and Hood
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
