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Annexin V/PI Staining Assay for Apoptosis
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MCF-1A0T cells were collected and resuspended in binding buffer at a density of 1× 106 cells/mL. After staining the cells with Annexin V-FITC/propidium iodide (PI) (BD Biosciences, San Jose, CA, USA) for 15 min in the dark. The apoptotic cell death rate was examined using the flow cytometry.
Copyright and License information: ©2020 Jiang et al.
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