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Follicle counting and ovarian morphological analysis
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Before staining with HE for histopathology, mouse ovaries were kept and fixed. The ovarian histological changes were observed by light microscopy (Olympus). We counted the follicles containing obvious nucleus in the experiment, which were divided into primordial, primary, secondary, antral, and atretic follicles, which were described before [3, 34].
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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