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Multilocus Sequence Typing (MLST) and Capsular Polysaccharide Serotyping

YL Yannan Liu
SL Sharon Shui Yee Leung
YH Yong Huang
YG Yatao Guo
NJ Ning Jiang
PL Puyuan Li
JC Jichao Chen
RW Rentao Wang
CB Changqing Bai
ZM Zhiqiang Mi
ZG Zhancheng Gao
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MLST of K. pneumoniae was performed according to Protocol 2 described on the MLST website1. The seven housekeeping genes (gapA, infB, mdh, pgi, phoE, rpoB, and tonB) of K. pneumoniae were amplified by PCR with primers in Protocol 2. Sequence types (STs) were determined by using the K. pneumoniae MLST database of Institut Pasteur2. To identify the capsular types of K. pneumoniae strains, the wzi allele was amplified, and the obtained sequences were aligned to the wzi sequences deposited in the database of Institut Pasteur (Brisse et al., 2013)2.

Copyright and License information:  ©2020 Liu, Leung, Huang, Guo, Jiang, Li, Chen, Wang, Bai, Mi and Gao.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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