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Body weight and food intake were measured once a week. After 16 weeks of the experimental protocol, 6 hr fasted mice were anesthetized with isoflurane and killed by cervical dislocation after blood collection through puncturing the orbital plexus. Blood samples were centrifuged at 1,200 g for 20 min at 4°C and serum was stored at −80°C. Adipose fat depots (ING and RP) were harvested, weighed, and processed as described below.
Copyright and License information: ©2020 The Authors. published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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