Paraffin sections

Seven μm paraffin cross sections incubated with CD45, as a marker of inflammatory infiltration, or either with OTX1 or OTX2 with the pan neuronal marker HUC/D, for double staining. Optimal dilutions of antibodies are reported in Table 1. Coverslips were mounted with Citifluor mounting medium and then observed with fluorescent microscopy (Nikon Instruments, Melville, NY, USA).

Note:

Supply companies: Amersham, GE Healthcare, Buckinghamshire, UK; Bioss antibodies, MA, USA; Cell Signaling Technology, Danvers, MA, USA; Invitrogen, Thermo Fisher, MA, USA; Merck Millipore Burlington, MA, USA; Molecular Probes, Thermo Fisher, MA, USA; R&D systems, Minneapolis, CA, USA; Santa Cruz Biotechnology, CA, USA; Sigma–Aldrich, Milano, Italy.