In vivo Toxicity Test

In vivo toxicity of hBD2 was assessed in 6–8 weeks old female NMRI mice (Taconic Europe). All animal studies were performed according to Danish legislations for laboratory animals and approved by Novozymes science ethics committee. hBD2 was given subcutaneously (s.c.) in the intrascapular region using a 25G needle and a 1 ml syringe. Animals were dosed on day 0 with the indicated amount of hBD2, applied as 10 mg/kg according to the individual body weight. Clinical signs were recorded on day 0 and monitored until experimental endpoints. Body weights were recorded as a minimum on day 0, day 2, and day 4 prior to euthanasia. Necropsy was performed after euthanasia and kidneys, spleens and livers were weighed.