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Apoptosis of PANC-1 cells was assessed by an Annexin V-FITC apoptosis detection kit (Thermo Fisher Scientific) on a flow cytometer (FACScan™, BD Biosciences, Franklin Lakes, NJ, USA). PANC-1 cells were collected in pre-cold PBS (Thermo Fisher Scientific) and then resuspended in 100 μL binding buffer from the Annexin V-FITC apoptosis detection kit. Later on, the cells were stained with propidium iodide (PI) and Annexin V-FITC for 15 min in darkness at room temperature according to the recommended protocols. Then, the percentage of apoptotic cells was analyzed.
Copyright and License information: ©2020 Wang et al.
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